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Dawley Inc vsmc growth
Vsmc Growth, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsmc+growth/vsmc/pmc13167699-0-68-47
Average 86 stars, based on 1 article reviews
vsmc growth - by Bioz Stars, 2026-09
86/100 stars

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Binding Assay:

Article Title: AP ‐1‐Targeting Decoy Oligonucleotides: Mechanisms and Therapeutic Potential as Modulators of Inflammatory Pathways
Article Snippet: .. AP‐1 decoy ODN , Vascular remodelling or hypertension‐related vascular disease involving abnormal vascular smooth muscle growth , Ds ODN designed to mimic AP‐1 binding site; often phosphorothioate backbone for stability , Transfection into cultured VSMC , VSMC cultured from rat models such as Wistar‐Kyoto (WKY) rats and Sprague–Dawley rats , AP‐1 decoy ODN blocks Ang II‐stimulated AP‐1 activity, leading to inhibition of TGF‐beta gene expression and reduction in VSMC growth , [ ] . .. CRE‐TF decoy ODN , Tumour , Phosphorothioate backbone , Cell penetration , Tumour cell lines , Inhibition of tumour cell proliferation; specific interference with CRE‐ and AP‐1‐directed transcription , [ ] .

Transfection:

Article Title: AP ‐1‐Targeting Decoy Oligonucleotides: Mechanisms and Therapeutic Potential as Modulators of Inflammatory Pathways
Article Snippet: .. AP‐1 decoy ODN , Vascular remodelling or hypertension‐related vascular disease involving abnormal vascular smooth muscle growth , Ds ODN designed to mimic AP‐1 binding site; often phosphorothioate backbone for stability , Transfection into cultured VSMC , VSMC cultured from rat models such as Wistar‐Kyoto (WKY) rats and Sprague–Dawley rats , AP‐1 decoy ODN blocks Ang II‐stimulated AP‐1 activity, leading to inhibition of TGF‐beta gene expression and reduction in VSMC growth , [ ] . .. CRE‐TF decoy ODN , Tumour , Phosphorothioate backbone , Cell penetration , Tumour cell lines , Inhibition of tumour cell proliferation; specific interference with CRE‐ and AP‐1‐directed transcription , [ ] .

Cell Culture:

Article Title: AP ‐1‐Targeting Decoy Oligonucleotides: Mechanisms and Therapeutic Potential as Modulators of Inflammatory Pathways
Article Snippet: .. AP‐1 decoy ODN , Vascular remodelling or hypertension‐related vascular disease involving abnormal vascular smooth muscle growth , Ds ODN designed to mimic AP‐1 binding site; often phosphorothioate backbone for stability , Transfection into cultured VSMC , VSMC cultured from rat models such as Wistar‐Kyoto (WKY) rats and Sprague–Dawley rats , AP‐1 decoy ODN blocks Ang II‐stimulated AP‐1 activity, leading to inhibition of TGF‐beta gene expression and reduction in VSMC growth , [ ] . .. CRE‐TF decoy ODN , Tumour , Phosphorothioate backbone , Cell penetration , Tumour cell lines , Inhibition of tumour cell proliferation; specific interference with CRE‐ and AP‐1‐directed transcription , [ ] .

Activity Assay:

Article Title: AP ‐1‐Targeting Decoy Oligonucleotides: Mechanisms and Therapeutic Potential as Modulators of Inflammatory Pathways
Article Snippet: .. AP‐1 decoy ODN , Vascular remodelling or hypertension‐related vascular disease involving abnormal vascular smooth muscle growth , Ds ODN designed to mimic AP‐1 binding site; often phosphorothioate backbone for stability , Transfection into cultured VSMC , VSMC cultured from rat models such as Wistar‐Kyoto (WKY) rats and Sprague–Dawley rats , AP‐1 decoy ODN blocks Ang II‐stimulated AP‐1 activity, leading to inhibition of TGF‐beta gene expression and reduction in VSMC growth , [ ] . .. CRE‐TF decoy ODN , Tumour , Phosphorothioate backbone , Cell penetration , Tumour cell lines , Inhibition of tumour cell proliferation; specific interference with CRE‐ and AP‐1‐directed transcription , [ ] .

Inhibition:

Article Title: AP ‐1‐Targeting Decoy Oligonucleotides: Mechanisms and Therapeutic Potential as Modulators of Inflammatory Pathways
Article Snippet: .. AP‐1 decoy ODN , Vascular remodelling or hypertension‐related vascular disease involving abnormal vascular smooth muscle growth , Ds ODN designed to mimic AP‐1 binding site; often phosphorothioate backbone for stability , Transfection into cultured VSMC , VSMC cultured from rat models such as Wistar‐Kyoto (WKY) rats and Sprague–Dawley rats , AP‐1 decoy ODN blocks Ang II‐stimulated AP‐1 activity, leading to inhibition of TGF‐beta gene expression and reduction in VSMC growth , [ ] . .. CRE‐TF decoy ODN , Tumour , Phosphorothioate backbone , Cell penetration , Tumour cell lines , Inhibition of tumour cell proliferation; specific interference with CRE‐ and AP‐1‐directed transcription , [ ] .

Gene Expression:

Article Title: AP ‐1‐Targeting Decoy Oligonucleotides: Mechanisms and Therapeutic Potential as Modulators of Inflammatory Pathways
Article Snippet: .. AP‐1 decoy ODN , Vascular remodelling or hypertension‐related vascular disease involving abnormal vascular smooth muscle growth , Ds ODN designed to mimic AP‐1 binding site; often phosphorothioate backbone for stability , Transfection into cultured VSMC , VSMC cultured from rat models such as Wistar‐Kyoto (WKY) rats and Sprague–Dawley rats , AP‐1 decoy ODN blocks Ang II‐stimulated AP‐1 activity, leading to inhibition of TGF‐beta gene expression and reduction in VSMC growth , [ ] . .. CRE‐TF decoy ODN , Tumour , Phosphorothioate backbone , Cell penetration , Tumour cell lines , Inhibition of tumour cell proliferation; specific interference with CRE‐ and AP‐1‐directed transcription , [ ] .



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A Representative western blot for tafazzin, complex I (CI) and citrate synthase (CS) <t>in</t> <t>hVSMCs</t> transfected with non-targeting control (ctrl) or siRNA against TAFAZZIN (siTAZ) or in mVSMCs overexpressing empty vector (EV), tafazzin (Taz) or transacylase mutant tafazzin (Taz H69Q or TazH) with quantification. Tub = tubulin. Data normalised to citrate synthase for tafazzin and CI or tubulin for citrate synthase ( n = 5, 2 male (M) and 3 female (F) <t>VSMC</t> donors). B Tetra-acyl cardiolipin abundance determined by mass spectrometry in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 5, 3 M, 2 F for all groups except EV, n = 6, 3 M, 3 F). C , D Representative Seahorse profiles of oxygen consumption rate (OCR) with sequential addition of oligomycin (oligo), FCCP and antimycin/rotenone (A/R) in control or siTAZ hVSMCs ( C ) and in EV, Taz, TazH mVSMCs ( D )( n = 3, 2 M, 1 F). E Maximal OCR after FCCP in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 6, 3 M, 3 F). F ATP/ADP ratio ( n = 4, 2 M, 2 F) and ( G ) MitoSOX mean fluorescence intensity (MFI) in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 6, 3 M, 3 F). arb. units = arbitrary units. H–J Percentage of cells positive for EdU ( H ), annexin V ( I ) and annexin V after tert-butyl hydrogen peroxide treatment ( J ) in siTAZ hVSMCs and in Taz/TazH mVSMCs (for H n = 6, 2 M, 4 F, for I , J n = 4, 2 M, 2 F.) For ( A – J ) n = number of independent experiments. For ( A , B , E–J ) two-sided unpaired t test for siTAZ vs. control comparisons; 1-way ANOVA, Bonferroni-Holm post hoc for EV, Taz and TazH VSMC comparisons. Data are shown as mean ± SEM with nominal or multiplicity-adjusted p -values. Source data are provided as a Source Data file.
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A Representative western blot for tafazzin, complex I (CI) and citrate synthase (CS) <t>in</t> <t>hVSMCs</t> transfected with non-targeting control (ctrl) or siRNA against TAFAZZIN (siTAZ) or in mVSMCs overexpressing empty vector (EV), tafazzin (Taz) or transacylase mutant tafazzin (Taz H69Q or TazH) with quantification. Tub = tubulin. Data normalised to citrate synthase for tafazzin and CI or tubulin for citrate synthase ( n = 5, 2 male (M) and 3 female (F) <t>VSMC</t> donors). B Tetra-acyl cardiolipin abundance determined by mass spectrometry in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 5, 3 M, 2 F for all groups except EV, n = 6, 3 M, 3 F). C , D Representative Seahorse profiles of oxygen consumption rate (OCR) with sequential addition of oligomycin (oligo), FCCP and antimycin/rotenone (A/R) in control or siTAZ hVSMCs ( C ) and in EV, Taz, TazH mVSMCs ( D )( n = 3, 2 M, 1 F). E Maximal OCR after FCCP in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 6, 3 M, 3 F). F ATP/ADP ratio ( n = 4, 2 M, 2 F) and ( G ) MitoSOX mean fluorescence intensity (MFI) in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 6, 3 M, 3 F). arb. units = arbitrary units. H–J Percentage of cells positive for EdU ( H ), annexin V ( I ) and annexin V after tert-butyl hydrogen peroxide treatment ( J ) in siTAZ hVSMCs and in Taz/TazH mVSMCs (for H n = 6, 2 M, 4 F, for I , J n = 4, 2 M, 2 F.) For ( A – J ) n = number of independent experiments. For ( A , B , E–J ) two-sided unpaired t test for siTAZ vs. control comparisons; 1-way ANOVA, Bonferroni-Holm post hoc for EV, Taz and TazH VSMC comparisons. Data are shown as mean ± SEM with nominal or multiplicity-adjusted p -values. Source data are provided as a Source Data file.
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A Representative western blot for tafazzin, complex I (CI) and citrate synthase (CS) <t>in</t> <t>hVSMCs</t> transfected with non-targeting control (ctrl) or siRNA against TAFAZZIN (siTAZ) or in mVSMCs overexpressing empty vector (EV), tafazzin (Taz) or transacylase mutant tafazzin (Taz H69Q or TazH) with quantification. Tub = tubulin. Data normalised to citrate synthase for tafazzin and CI or tubulin for citrate synthase ( n = 5, 2 male (M) and 3 female (F) <t>VSMC</t> donors). B Tetra-acyl cardiolipin abundance determined by mass spectrometry in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 5, 3 M, 2 F for all groups except EV, n = 6, 3 M, 3 F). C , D Representative Seahorse profiles of oxygen consumption rate (OCR) with sequential addition of oligomycin (oligo), FCCP and antimycin/rotenone (A/R) in control or siTAZ hVSMCs ( C ) and in EV, Taz, TazH mVSMCs ( D )( n = 3, 2 M, 1 F). E Maximal OCR after FCCP in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 6, 3 M, 3 F). F ATP/ADP ratio ( n = 4, 2 M, 2 F) and ( G ) MitoSOX mean fluorescence intensity (MFI) in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 6, 3 M, 3 F). arb. units = arbitrary units. H–J Percentage of cells positive for EdU ( H ), annexin V ( I ) and annexin V after tert-butyl hydrogen peroxide treatment ( J ) in siTAZ hVSMCs and in Taz/TazH mVSMCs (for H n = 6, 2 M, 4 F, for I , J n = 4, 2 M, 2 F.) For ( A – J ) n = number of independent experiments. For ( A , B , E–J ) two-sided unpaired t test for siTAZ vs. control comparisons; 1-way ANOVA, Bonferroni-Holm post hoc for EV, Taz and TazH VSMC comparisons. Data are shown as mean ± SEM with nominal or multiplicity-adjusted p -values. Source data are provided as a Source Data file.
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A Representative western blot for tafazzin, complex I (CI) and citrate synthase (CS) <t>in</t> <t>hVSMCs</t> transfected with non-targeting control (ctrl) or siRNA against TAFAZZIN (siTAZ) or in mVSMCs overexpressing empty vector (EV), tafazzin (Taz) or transacylase mutant tafazzin (Taz H69Q or TazH) with quantification. Tub = tubulin. Data normalised to citrate synthase for tafazzin and CI or tubulin for citrate synthase ( n = 5, 2 male (M) and 3 female (F) <t>VSMC</t> donors). B Tetra-acyl cardiolipin abundance determined by mass spectrometry in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 5, 3 M, 2 F for all groups except EV, n = 6, 3 M, 3 F). C , D Representative Seahorse profiles of oxygen consumption rate (OCR) with sequential addition of oligomycin (oligo), FCCP and antimycin/rotenone (A/R) in control or siTAZ hVSMCs ( C ) and in EV, Taz, TazH mVSMCs ( D )( n = 3, 2 M, 1 F). E Maximal OCR after FCCP in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 6, 3 M, 3 F). F ATP/ADP ratio ( n = 4, 2 M, 2 F) and ( G ) MitoSOX mean fluorescence intensity (MFI) in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 6, 3 M, 3 F). arb. units = arbitrary units. H–J Percentage of cells positive for EdU ( H ), annexin V ( I ) and annexin V after tert-butyl hydrogen peroxide treatment ( J ) in siTAZ hVSMCs and in Taz/TazH mVSMCs (for H n = 6, 2 M, 4 F, for I , J n = 4, 2 M, 2 F.) For ( A – J ) n = number of independent experiments. For ( A , B , E–J ) two-sided unpaired t test for siTAZ vs. control comparisons; 1-way ANOVA, Bonferroni-Holm post hoc for EV, Taz and TazH VSMC comparisons. Data are shown as mean ± SEM with nominal or multiplicity-adjusted p -values. Source data are provided as a Source Data file.
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Image Search Results


A Representative western blot for tafazzin, complex I (CI) and citrate synthase (CS) in hVSMCs transfected with non-targeting control (ctrl) or siRNA against TAFAZZIN (siTAZ) or in mVSMCs overexpressing empty vector (EV), tafazzin (Taz) or transacylase mutant tafazzin (Taz H69Q or TazH) with quantification. Tub = tubulin. Data normalised to citrate synthase for tafazzin and CI or tubulin for citrate synthase ( n = 5, 2 male (M) and 3 female (F) VSMC donors). B Tetra-acyl cardiolipin abundance determined by mass spectrometry in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 5, 3 M, 2 F for all groups except EV, n = 6, 3 M, 3 F). C , D Representative Seahorse profiles of oxygen consumption rate (OCR) with sequential addition of oligomycin (oligo), FCCP and antimycin/rotenone (A/R) in control or siTAZ hVSMCs ( C ) and in EV, Taz, TazH mVSMCs ( D )( n = 3, 2 M, 1 F). E Maximal OCR after FCCP in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 6, 3 M, 3 F). F ATP/ADP ratio ( n = 4, 2 M, 2 F) and ( G ) MitoSOX mean fluorescence intensity (MFI) in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 6, 3 M, 3 F). arb. units = arbitrary units. H–J Percentage of cells positive for EdU ( H ), annexin V ( I ) and annexin V after tert-butyl hydrogen peroxide treatment ( J ) in siTAZ hVSMCs and in Taz/TazH mVSMCs (for H n = 6, 2 M, 4 F, for I , J n = 4, 2 M, 2 F.) For ( A – J ) n = number of independent experiments. For ( A , B , E–J ) two-sided unpaired t test for siTAZ vs. control comparisons; 1-way ANOVA, Bonferroni-Holm post hoc for EV, Taz and TazH VSMC comparisons. Data are shown as mean ± SEM with nominal or multiplicity-adjusted p -values. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Defective vascular smooth muscle cell tafazzin impairs mitochondrial function and promotes atherosclerosis in preclinical models

doi: 10.1038/s41467-025-65873-y

Figure Lengend Snippet: A Representative western blot for tafazzin, complex I (CI) and citrate synthase (CS) in hVSMCs transfected with non-targeting control (ctrl) or siRNA against TAFAZZIN (siTAZ) or in mVSMCs overexpressing empty vector (EV), tafazzin (Taz) or transacylase mutant tafazzin (Taz H69Q or TazH) with quantification. Tub = tubulin. Data normalised to citrate synthase for tafazzin and CI or tubulin for citrate synthase ( n = 5, 2 male (M) and 3 female (F) VSMC donors). B Tetra-acyl cardiolipin abundance determined by mass spectrometry in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 5, 3 M, 2 F for all groups except EV, n = 6, 3 M, 3 F). C , D Representative Seahorse profiles of oxygen consumption rate (OCR) with sequential addition of oligomycin (oligo), FCCP and antimycin/rotenone (A/R) in control or siTAZ hVSMCs ( C ) and in EV, Taz, TazH mVSMCs ( D )( n = 3, 2 M, 1 F). E Maximal OCR after FCCP in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 6, 3 M, 3 F). F ATP/ADP ratio ( n = 4, 2 M, 2 F) and ( G ) MitoSOX mean fluorescence intensity (MFI) in siTAZ hVSMCs and in Taz/TazH mVSMCs ( n = 6, 3 M, 3 F). arb. units = arbitrary units. H–J Percentage of cells positive for EdU ( H ), annexin V ( I ) and annexin V after tert-butyl hydrogen peroxide treatment ( J ) in siTAZ hVSMCs and in Taz/TazH mVSMCs (for H n = 6, 2 M, 4 F, for I , J n = 4, 2 M, 2 F.) For ( A – J ) n = number of independent experiments. For ( A , B , E–J ) two-sided unpaired t test for siTAZ vs. control comparisons; 1-way ANOVA, Bonferroni-Holm post hoc for EV, Taz and TazH VSMC comparisons. Data are shown as mean ± SEM with nominal or multiplicity-adjusted p -values. Source data are provided as a Source Data file.

Article Snippet: Aortic and plaque hVSMCs were cultured in VSMC medium (PromoCell GmbH, SMC-GM2 C22062 ) with 5% Foetal Bovine Serum, supplemented with 100U/ml penicillin, 100 μg/ml streptomycin. hVSMCs were passaged when 90% confluent, reseeding at 13,333 cells/cm 2 , and studied at passages 2–8.

Techniques: Western Blot, Transfection, Control, Plasmid Preparation, Mutagenesis, Mass Spectrometry, Fluorescence